一口吃不成胖子﹐ 一步跨不到天邊。

Rome was not built in a day
~ An English idiom

The next focus for my new goldfish variety project is to construct the dragon eyes from the F1s. Since the F1s have recessive alleles for dragon eyes (Ee), I select only those with pearl scale features for inbreeding and linebreeding across different batches to create F2.

The Punnett chart for F2:

Very few (only 25% theoretically) of the F2 exhibits the dragon eyes (ee) features. The normal eyes F2 will not be used for next propagation as there is no way to tell if it is a pure normal eye (EE) or a normal eye that carries a recessive allele for dragon eye (Ee).

From the thousands, there are even fewer of those with both dragon eyes and pearl scale features. In order to get better breeders, I did numerous spawns using breeders from the F1 sublines just to get a few suitable F2 for the next propagation.

The problem of incomplete dominance for pearl scale seems prevalent even in the F2 as not many have good pearl scale properties.

Pictures:
1) F2 - in this batch, only 1 has dragon eyes (circa 2006).